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CRIMIC CR71313

Gene Wdr81

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12014303..12020960	33B8-33B9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ATATGGGGGGTGCTCGAAATTGG
sgRNA2 sequence	TTTATTGGGTTAGGCGGGTTAGG

 

left_200bp	right_200bp
CAGCTGATTCCTCAAGTTCGCGAAAAAAAATAGTAGCATTCTTTGATAAGATAAGAAAAAATTATCGAAACACCCAACAAATCGCCGAGCTTAAGCCCAAAAAGCCATGGCTGAATAAGATCGAACCTCGCCCCAGTCGGAAAATGTGATCGAACCGCGGTGGCAGGCAGCAGCGGATTGAAATCGCAAGATTGCCAATT	CCGCCTAACCCAATAAATTAATGTATTGAGGTAGCGCTTGCATTGCTATTATTATTTATTATAATTACGATCGCACATTTATAGCTACTTTCTTCTAGCATAATCAAAGCATTCCAGGTCCACAATTTATATGTATTTATATATATATGTATGTCCATTACAATAGAAGACATAATTGTATTTAAAAGTAACTACTTAAG

 

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