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CRIMIC CR71316

Gene Baz

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:17160006..17200728	15F1-15F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTGCAGCTCGTCTCAGTTTGCT	TGCACCATCTGCACATCTGTGCTT

Images

Construct Design Sequences

sgRNA sequence	TTCTCCAAGTGAGTCGTTGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAATAAACCAGCAAAAGATAAAAATCAATAGCATACAGATAAACAGAAACTCCGGGTGAATGAAGCAATTTAGATAAATTAATCCATTGAGTTCCTATGCTAGAGTTTTCGAAACATTTAGTAAACCCATAATGAAATGTTGATCTTAGATTTGTAAAATCTCCTCCAGTTCTTGCTAGAATTTTCTCCAAGTGAGTCGT	TGTTGGTGGTGGTCGGCATTTGGCATTTGTTGTGGAATCCATCGGAATGCCAAAAGCAACACCGCCACCAGCTCCTTGGCTGCTCAGCTCAGCTCGACGAGAAGAGTAGATTGCAATTTGGGGCGCTGGAGGTCGTCGTGGTCTTATTAGGCTTATTAGGCGTCGCTCAGGGAGTTCAAGTTGATGATGAGCCCACGTGA

 

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