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CRIMIC CR71317

Gene CG32099

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:12149273..12150329	68F6-68F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACAAAGCACGACTAAACGGTGAG	ACCCTTGCGGATGGTTATCCTGTT

Images

Construct Design Sequences

sgRNA sequence	TTCGCTATTGTGATGCAGTATGG
sgRNA2 sequence	TACAAGCGATCATGATGTATAGG

 

left_200bp	right_200bp
TATACAGGTTATTAGATTGCTTTTTTTAAATGCTACATATAGCTATCCCATTTTTTTTTTTAAATTAAAGATAGTCATTATTTCTTAATTTTTTAAAATGAAAATGGGTACACCCCGCATTTGGTGGAGCAGTATATTTGCAGTATATTTATAAGGCGTAATCGCTGGGGGATTTTTACCCTCTCCAGTTCTGGCCATACTGC	TATAGGAACTATAAGGAATCTAAGCCAAGCAAACGTAGCTCCTAAAAATAGCGTTATCCCTAGAATAAACATGCATTTATATTTTCTCAATGGTTGTGTGCCAAATTTGGAATAGTTTGTAATCCTTGTGTTGTCATGTTGCGCAGCGCTCACACCCCAACAAAAAATCGTTTGTTTGCCTGGCACTGGATAAACAAACG

 

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