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CRIMIC CR71318

Gene yip2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:9915253..9917220	30E4-30E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATCGCGACCAGAAGTCGAAAGT	TGTCCACTTGCTCACCCTTAAGAC

Images

Construct Design Sequences

sgRNA sequence	AAGTGCAACCGGCTGGACGCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTACTTGCATTAATTTATTTGTTGTTTTTTTTAAGAGTGAACCCACCTTAGAAAATGTCTGCCGCAACCAAAGGTAATTATGTGAATCCTTTAGGATATGCCTATGCTGTTGGAAGTCTAGATAAGAGATGATCTCGCTAGAGAGATCTGAACTTTGGACTCGTTGGTGTCTTATCTGATGCTATCCCCCCCCCCCGCG	TCCAGCCGGTTGCACTTTGGACCTGTTGAATCGAAAACAAAAATCCGCCTTCGTTCGAGCTAAATTTGTGCTATCACTACGCACATTATATATGTTATACATATGTTCGTATGTTTCTTCTCCTTTGTAAAGGTATCTATATTGTGGCTGCCAAGCGTACGGCCTTCGGAACCTTCGGTGGATCCTTGAAGGGCATCAAC

 

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