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CRIMIC CR71319

Gene Arc42

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:19877737..19879309	92B4-92B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCCCTCGTTTGGTCTGCGTTCTT	ATGCTAACCGACAAAGCCTTGCTG

Images

Construct Design Sequences

sgRNA sequence	GTGTGACCAGAGGAGGTCACCGG
sgRNA2 sequence	ATCCAGCGACTGGTGATCGCGGG

 

left_200bp	right_200bp
GGCGGCATGTTGGATCTTATTTCCGTGCACCTTACGAATTATGTTTATTTCATTTTAAAAGTACCCATCAGTGTTTTCATATGAGTGAAAAGTTAGTAATTTTTAATTTTAATTTTAAATTAGTAACAAGACGACTTGTTAAATTCGGGACAAGAGTCACCCTGTGATGAACCACTTGTTACATGCAGATGCAGCCGGTG	CGCGGGCTCCATTCTAAAGGAATATGCTAGTTAAGATCAAAGTTATATATCCGTACTACTTGTGTCTCGTTTAATAAAGAACGTTTTTAATATACTATTTTGCTAGTTCTATTGAGTTGTATAATTGTTTATAATAGATAGAAATACATGAAAAAAACACCTACAACATTTTAATTCTTTAGAAAAAGTTAGCAAAACAA

 

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