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CRIMIC CR71320

Gene Acat1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:7887130..7888785	7C1-7C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGACTATGAGCGTTGTGATGTGG	ATATGCGAACTTCTGGCCTCCAGA

Images

Construct Design Sequences

sgRNA sequence	TTGCGTAACAAACCGGTAATCGG
sgRNA2 sequence	TTAAGTGTCTTGATTGCGCGTGG

 

left_200bp	right_200bp
GCTGGGTATTGTAAAAAGAATATATAAACTTAGCATTGCATCTTTACAGCGTAGTTTTCAAGCAATATACTTAAAACAGCAAAAATTAAAAATTACATTTAAAGAATGCTAACTAATAGAAAATTACCAGTCGCTTTAATCTTATTTAACAGTTGCCAACACTTTTACCGCGCCAGCTGTTGCCTATCGATAGACCGATT	GCGTGGCCGCATTTAACGAACTATTGTGATTTGCATAATAAAATTATGAAACAGTCAATAATTATTATATGTATATCAAATCAGTGTTCTTCCATGACGGTAATAGGGTTCAAAGTCTAAACATCTTATATTTGTTCTGTGAAAAAAATGAATTAAGTGCTGAAAATGTTCTTTATTGTTGAATTTATATATATATATAT

 

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