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CRIMIC CR71321

Gene Ssadh

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:25624805..25626924	96E6-96E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGGTGGATTCCAGCAATGCAAAG	TCCTCTGCAAACCACTCGACGAA

Images

Construct Design Sequences

sgRNA sequence	TATATGATGGTCTTTATCTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGCGGAATCCTGCCAATGGTGCTGTCATCGGCAAAGTGCCCAACATGACGGTGGCGGATGCCCAGAAGGCCATAGACGCCGCTAAGCAGGCGTACGAGTCCAAGGAGTGGCGATCCCTGACCGCCAAGGATCGGTCCAACCTGCTCAAGGTGAGTGCCGGGATCACTCGTTATCAGGGGTGCTGATTGTTGACCCCACAG	ATAAAGACCATCATATAATATGATGGGACTATAGAGTTGGAAAGTTCGACTGTAGGTCATTGTCATTATTTCTTCCTCTGGTCTCCAAGTTACTTCTGCTTATCTAATCTAATCTCGTCTTGCAGAAGTGGCACAAACTGATCGAGCAGCACTCGCAGGAGATAGCCGAGATAATGACGGCCGAGTCGGGCAAGCCGATC

 

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