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CRIMIC CR71322

Gene Arfip

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:11231419..11235013	86D8-86D8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGCGCAATCTCACCAAG	CATGCGGTAGGCATCGAATTC

Images

Construct Design Sequences

sgRNA sequence	CATTTCTGATAAGAGCTGTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATTTCGTCGGTGAACACACTGTGCAACAAGACGATCGACGATACGCTGCTGACGATACGACAGTACGAAACGGCCAGGTAACCAAAGCTATATCCTTTCATTGTCGTGCAACAACGAAGGAAAAATCATCATCGATCTCTTCGATTTTAATTTAAACTCAGCATGTGTGTGGCTTTTTATAAAAAGTGTTGTGCCACAC	AGCTCTTATCAGAAATGCATTTTAAACTAGCCTTGTATACAAGCTTGAAACATTGCTTATCGTATTATTTCCTTGAAAAAATTTAAATACGTTGGATCAATTAGTCATAACCGCATTCCATGATAATCTCTACAATGGCAATAAGTTTTTATTTCCTAAAGCCATCGTATGTTTCATAAAAAGGCATGAGAATTATATGT

 

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