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CRIMIC CR71324

Gene obe

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:15486050..15493310	88F6-88F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGTTTGTGCGCTAGTCAGAG	GCTTACTTTGTCATCACCCAACTTAG

Images

Construct Design Sequences

sgRNA sequence	AGCCTGCCCACTCTGCGCTTTGG
sgRNA2 sequence	TCATGCATGTAGGTGCTACATGG

 

left_200bp	right_200bp
TTAAGTATTTAGTCATTGCACTTAATTTTAATGCCGCAGGCGTATTTGTAACGGTCTTCTATTTGAATTTAAACTTACGATATTCGACATTCAACATATTTTCCAAAGTGTGGCAACACCAGCAAGTTTCCTTCATCAGTGTGACCGCTTTTAGTATTTTTCAGTATTTTCGGTGGAAGAAGAAGCCTGCCCACTCTGCG	AGCACCTACATGCATGAATCACTTTCTATGAATTTATGAAGTAACTATTTTTATTGTGTAAAGTTAATAAAATTTGTATTGAAAGCGCAGCTATGTTTTTCAATTTAATTTTTTTATGTGATCAATATAAATACCACTTAGCCATCCATAACTATCGACATGTTAACTACATTTATTCTACGTGGCACAGTGGCGGCACC

 

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