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CRIMIC CR71325

Gene CG6230

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:11115635..11120319	32D4-32D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTGGTCTAAGTAAAACAGAAGCTG	CTCGAAAGTAGAGGATCCTGTACC

Images

Construct Design Sequences

sgRNA sequence	CACAGGAAACGCGCTGCAGTAGG
sgRNA2 sequence	AACGAAAGGGGTTTCTCTAGTGG

 

left_200bp	right_200bp
GAATTTGAATTTAAAAATCCGATAACATATCGCTGACACTGGATTTCCAACACTAAATCGGCGCTATCGATAGCATAAGACAAACAGCTGGTGCCTGTCAACGTTGCTTGAATTTTCACAATTTTTTTTTGCCGCTATGACATTACAACTATAAAAAGTGCTTTAGATTTTCTAAAAACCTCACACAGGAAACGCGCTGC	GAGAAACCCCTTTCGTTTTTATTTGTACCCCCATAATGATGAATAACAAAACGTAATATTATGGCGTCGGTTTCATAAATATTGAAATTCTTCATCTAAGAATCAACATAATGCATTTAAGGTGTATTATCAGGCAAGTGTATTATCGGGCATCCTATATCATTTTATTTTGACAAATTGAGATCTTTCGATTTTTTCGA

 

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