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CRIMIC CR71326

Gene CG5276

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11431738..11433543	86E5-86E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	AGCACGTGTGCTGCTGAAGAAGAA	CCACCGGCTTTGTACGTT

Images

Construct Design Sequences

sgRNA sequence	CTGATCGCCCGCTCATGCGCTGG
sgRNA2 sequence	TGGAGTGCGGTAGCTTGGAATGG

 

left_200bp	right_200bp
ATAAATTGCAAGTGACTGCGATACCAGTTGACAGTTGTATTGTTTTGATAATTTCAATTACCTTCGGTCTTTGTCCAAATCATGTTTTTCCCAGTATGTTTACATTAACTTAAACTGAATTGTTGCACCTGTTTCTCGATAACATCGCATGCGCTCGCCAGTTTGCACCAGCTGTTGCGCCAGCTGATCGCCCGCTCATG	CAAGCTACCGCACTCCACTCTACTTCGATCCAGGTGCTTCAGTTTTAAACGTGTACATATTGTATTATTGTCCCATACGGTAAGCTTTATATTTATAGTCACCTAGATTAGTCCAGAGATGCTCATCTGCCTCATGAACCGTGACTCCTTGTCCCGTCTTGTACCATAGCACAAAAAAAAATTTTAGTTTATTTTTATTA

 

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