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CRIMIC CR71327

Gene cpb

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:1704902..1706962	22B1-22B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAACAAGGGAAAGCAGAAAGTACC	GCTCTTTAGTCCAGTCCAAGCG

Images

Construct Design Sequences

sgRNA sequence	GCATTTCCGACTGAGAAGAGAGG
sgRNA2 sequence	GAATCGAACTGAGTACTGAGCGG

 

left_200bp	right_200bp
AAGCCAAAGATTCTTGGCAATCACCTAATCCACCGAGAAAACCTCCAGAGGGGCGGCGGCCTGTGGCCTCAAATAATGCCCATATATGGTAATCCCTTGTTTGCCGGGTGGCAGTGGAAAGCGGAGGGATTTTTTGGGCTCTCTTATCGGCGGTGGTGCAGTGACTCAATTTCAATTTCACTGGTGTCTCTTCTCCTCTC	GAGCGGCTGAAGGCTGAATTTCCACATAAACCAATCCAGTCCAGGGGCGTCCCACAAGTAGGCTGCTCCCAAGAAGAGCGCAGGAATATGCGAATTGCAAGGCACTGTCGTCGCCACTAAATATTGTCATAAATAGAAATAATTTTCATAATATTTCGGCTGCACCCACCCAATTCGCTGTTACTCGCCCAATTGTGTTG

 

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