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CRIMIC CR71329

Gene twin

CG31137

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:24196893..24221965	95F1-95F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGATACGGCACTATGCAGCG	ATGGTGGGTACAAGAGCTGAG

Images

Construct Design Sequences

sgRNA sequence	ATGCAGCGATCTTAATAAACAGG
sgRNA2 sequence	TCCGACCCCTATATCCGCCCGGG

 

left_200bp	right_200bp
GGAGCAATTCTATCACTTCTTCCTGCCGGAACTCAAGAACGATGGGTACGAGGGAATCTTCTCACCGAAGTCGCGTGCTAAGACTATGTCCGAGCTGGAGAGGAAGTACGTCGATGGCTGTGCGATATTCTTCAGGGCGTCCAAGTGAGTACACATGTCAATCCCTATTTCAATGATAAACTTATGCAGCGATCTTAATA	CGGATATAGGGGTCGGAGCTGCAGCAATAGCGGATGCCATCGTCTGACGGCTGCTCTCGGAGTGGCAAGAAAAGCAGTGAATCCTCCCCCGCCGCCGCCGGCGGATAAGCAGCAAACCGCCGCTCCCCATCCCGCTCCCAACTGACGTGGCGATCTGCTCGCATCAAAATTCAGTTTAAGTTAGATACGACAAACTTTGA

 

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