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CRIMIC CR71330

Gene COX4L

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:7693727..7601601	28C1-28C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCTTCTTTGAGCTGACATGACAGC	AGTCAAAGGAACACAGGACACG

Images

Construct Design Sequences

sgRNA sequence	CAACACAACTGTCGAATTTCCGG
sgRNA2 sequence	ACGTTTCTGTACATAGTTTACGG

 

left_200bp	right_200bp
AATTTTTGCCACGCCTTAAACAATTTTTAAGAAGAAGTAAAATTTTCAGACTATCTTAGTGCTCAACGAAGAGTGCAATTCAGAACTTAAAAAGTACATCTAGTTTGTAGATAAGGAAACTGTCATATTTTTTTTTGTATTCAACAAACAGACTAGAGAAATTTCATTTTCATTCGACACGAGCAACACAACTGTCGAAT	ACTATGTACAGAAACGTAACTAGCAAAATACAATTCAACAGCAAAGTCGCTTGCCATTTGATCGTTACGTGCTGAATCGGGCAAATACCCAATATCTATAGATTTTTGTGTCTCTAGCTGTGTAACTCGACTATAGCATTTCCTCCCGTTTGAAATTAGGGTGTGTATGTAAATTCTCAGACACAACTTAATTTAGTGTT

 

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