CR71332

CRIMIC CR71332

Gene Dbp21E2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:811584..813380	21E2-21E2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGGATGTATGATGAAGCAGCAG	CGACATCCTGATCGCCAAACGAAA

Images

Construct Design Sequences

sgRNA sequence	CGTGTGCACAGCTGACTTGCCGG
sgRNA2 sequence	ACTAAATGGACATACGCCTCAGG

left_200bp	right_200bp
CTAATAAATGATTCGTATGTGCAGGAAGAAATGTAATTTTATAAAAAAAAACAGATTATTATTATTATTATTATTATTATTATTATTATTATATTAGTTGCTGGGTCTTGAAATAAATATGAATTTCAAACTTACCGCCACAAGTGGAATTGAATTCGAGCGGCGCTTTCTAACACTTCGATGCGTGTGCACAGCTGACT	CTCAGGAAGAAGCCTTTTAGTTCTGTGTTTTTATGTATTACGAGTAAAGTTGTATCACCTTTCGGAAGACCGAAGAGGAAGTCTAAAGTAAACGATGAGTTTTATTGAGATCCCCAGGTCCTCCTGGACCTCCAGAAGGTCTTATAATACTTTCCCAAGTCCCTTTCAGATCCTTAGGTAGTGATAGCCACTGATGGGCG