CR71333

CRIMIC CR71333

Gene CG31937

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:1729887..1731070	22B1-22B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAAGAAAGATAGTACTACCCTTACCTAGC	CTATGCATCTGAACCAGATGATAGATTAC

Images

Construct Design Sequences

sgRNA sequence	TCTGTATGGCACGGCTGGGTCGG
sgRNA2 sequence	GGGAAGGGGCGAGGCACACCGGG

left_200bp	right_200bp
TATTTAAATAATATAGCATCATTCAAAAACCAAGTATAAGGGCTATAAGTCAGATAAGGTGACTACTTTCCGCAGTCACTGCGTCACCTCTGGTGCCCACCAACAGCTGATCGATCAGACCCGCTCGCAGTGATTTCTGTGTGGCCAAGAACAGGTTTCAGTGCCTTCCGTTGCGGCAGAGGACAAAGGTTTCTCCGACC	GTGCCTCGCCCCTTCCCTATTCCTGCTCCAAACGGCTCTTAGTCATCTTTTATACTGTTGTATAATTCTTAACTATATACTAAAATATATAAATATGTGCACAAGATTAAGTTGAGTGTGCGTTTCAATCGCGATAATCTTCACAGGCACAGTGGGATTCTACAGTTCCTCCCATTCAATTGGTGAGCAATATTGTGGTA