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CRIMIC CR71334

Gene kz

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:2237858..2242035	2F1-2F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACACCATTGCAGGTAGCTGGATT	GCCACATGCCCAATTAGCCAAATC

Images

Construct Design Sequences

sgRNA sequence	TTGGTAAAGTAAAGTAATCATGG
sgRNA2 sequence	TAAAAAAGCTAGATAAAAGGGGG

 

left_200bp	right_200bp
CAGTTTTTCCCACTAATGTATGTTTCCACTAACACAAATGTAACATCACAGCACATTTTAGAGTATCTCAACTTTTGTTATCGCCTGCGCTGTTATCGATAAGCGTACTTCGCACGTGTGATTTAAATCAATGTTTATATTATTGCCCAAAGTTAATTCTGTTACTTTATTTTATTAACTATATTGGTAAAGTAAAGTAA	AGGGGGTTTTTTTGTATAACAAGTTTCATTTATTATTTATTTAATTGATATATATTTATATAATTTTTATCTTTAATATAAATATGAAATTTGTTTTAAATGGAACTAAAAAAATGGAGTGGAGTGAGGGGGAATAAAAATGTTTTGTTAACAGCTAGTTTCGATAAATGTTTAATACAATCCATATATGTACATAGAAA

 

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