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CRIMIC CR71335

Gene eIF2Beta

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:16445176..16446993	14C6-14C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAGCGGAGACATGTAGGACAG	AAGATGGGTCTGGACGCTTTTCAG

Images

Construct Design Sequences

sgRNA sequence	GTGACAGCACGGCAATCAGACGG
sgRNA2 sequence	CTGTGGCTCCCGCTGCTCGGTGG

 

left_200bp	right_200bp
AAACTCGAGTTGAGTTATTGAATGTTAACTGTTCGTAGTTCGAAAGTTATCTAATTTAGCTAACTAATTTAGCAATTGTTTATAAAAAAGTTTTACTTGAATATTATTAATACTCGGTATATCTATGCAAATGGTATTAAAAGTATATTTTCGCATGCCGGATGGTTTACTCGTCTAAAACTCAGCCGGTCACACCGTCT	CGGTGGCCAGCATTAAGTCAGGTTTCCAGGCTGTCACCGGCAAACGTGCCGCCATACGAGCAAAAACAACCTAAATTCTCCTTATTTAAAACTTTTTGAATTTTTATTACCAGTAAAATTTCAATACAAAAAACCAACGATACTATTTATTTGTTGTATTCTGCATTGTTTTATGCCTTCGTTGCCAGTCGGTTAGGTAG

 

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