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CRIMIC CR71337

Gene Exo70

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:8411851..8415910	66C11-66C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACACAAACATCTACGCGACGGAAC	CAAAGATCGGAGTAGAGCATCGC

Images

Construct Design Sequences

sgRNA sequence	TAACCGAAACGATCAGAGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGAAGTGGTTCTCCGATCGCTGCAGCTGCTCAAGGATCACCAAAAGAGCAACAGTTGGGGTCACGAGGCTCTGGTGAGTAAACAGTTCTTTCATATTCTAACTAGTATTAATACATATATATTATGTGGAGAAAAATAGTACGTAGTAGAATATGAATAGCGATGTGTACGATGATGAGCATATGTATGCTTTTCCCATC	TCTGATCGTTTCGGTTAACGGTGTCTGTTCGAAAAAGCTTGTGCATGTAAGCATTACATGTCCAATTCGATGACCGCCATTTGCCAGCTTGAATTCGCAAAGAACGCATCATTTTCTGTGTCACAGTATCTAAAAAAAATGGAGAATTCTCGCACGCCCACGAACAAGACCAAAATTACGCTTAATCGCACGCCAACGCT

 

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