CR71338

CRIMIC CR71338

Gene mri

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:200174..202152	61B2-61B2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AAACTATCGACTGAGCGCAC	TTCACTGAACTGGGAGACTTCGAC

Images

Construct Design Sequences

sgRNA sequence	GTACTGGAATATTTGAAGAGTGG
sgRNA2 sequence	AATGCTCAACGAGTTGGACCAGG

left_200bp	right_200bp
TATGTATATTTATAACTATATGTAAAAAGGAAAAAGTCGCGTCGCACAAAGGTAACGTGAATACTATTTAATCGGACCTCTGATGCAGCGATAAGCAGGCTTTTTTTCAACGGAGCCTCGCTAATTTGACCGCCCTCTGCCAGATTGTCTTTGTCTCTCTCGAAAAGTCGGTGGAACGGTGTTGTACTGGAATATTTGAA	ACCAGGCAGCGATCGCTGTTGCCGTCGGAATAGTTGATGAAAATATGTAGATCAACTGACCGGCTGGTAGTCGGAACACACTGCCAGAGCATCACAGCGCACAGAAAAACACTTTACGTTTTTAATTAACATTTTTATATGTATATGTAATAAATGGAAATTTCGAATACGAAAAACCAAGCAATTGATTTACGAATAGA