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CRIMIC CR71339

Gene Hap40

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:15704125..15705806	13E8-13E8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	GCCATGAGCACCACCAACTG	GCCGCTAGGAAGGATCTAGCTG

Images

Construct Design Sequences

sgRNA sequence	CAAACTATGTTTAGAAATACGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTATCAGTACCCCAAAAACCTGATCGAGCAATATCTAAGGGCCTCCAGCAAGATCAAGAAGTTCGAGCGGGCCGGCTTCTTCAAGTGAGTAGCTACGGCCCAAGTATGGGCAATCAGATACCTTGCAGATCATTGCGTTTGATTAGCAAGTCATTGCCCAATTTATCGCTTTTCCTCCATCTTCAAACTATGTTTAGAAA	TACGGGTGTATTAGAAGCCCTCGAATCACGGAATACAATCTCAATACTCCACTGTTTCTCTAATTCCCAGACGCTTTGCGCCCAGCGTGGTCGATGTGCAGGCGGACTTCCAGCGACTGGCCTACAGCTTCGAGGAGTCGGGCATACAGCAGTATGCGGCCATGTGCCACATTGGCTATGCCAAGTGCGAGTCCTACGGC

 

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