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CRIMIC CR71341

Gene egg

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:24775534..24779901	60E1-60E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGCTCATCGAAGATGAACTCGA	GTGGATAGTGTGACCGTTCGGATA

Images

Construct Design Sequences

sgRNA sequence	GGAACTTCATGCTGAGTAAATGG
sgRNA2 sequence	TAATGAGTATATTTCCTGTATGG

 

left_200bp	right_200bp
GCAGACCACTTGGAGCGACAATTCGCACTGGCAGATGTGGAACTCGAAAAAGTTCAGACCACCGCTGACAAAATGCACTGCGCTTTGTATAACTCGTGTCCGGTGGCACACAAACATCTGCCCACCCTGGACATTGAACCCAGTGACTATGTGGTAAGTAAGAAGTGATGCCGTTTGGATCTGATTTAATACAACCATTT	CTTCCTTGTACTTTACTTTTCCGCCAGTACTCGAGCAAAATTAAAAACTTTTGCCCGGCTGCACGGACTTCGTTTCGTTGTTTGTTCAACGGCAGGACCTCTCCTCAACCGCCTCACCTCCCGACTTACCCCCATCTTGACCGTCACTTCCGCCACTTAAGTTGTGTTTTATTTTACCCGAGCTGTGCTGCGTTTTTTTT

 

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