CR71342

CRIMIC CR71342

Gene Set1

CG40351

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:25107594..25225180	80F9-80F9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGATTCGTCTCTGGCAAACTC	GTTCGTGTTTACAGATCAAATGGGTTTTAG

Images

Construct Design Sequences

sgRNA sequence	GCTATCGATGACAAACCTATGGG
sgRNA2 sequence	AAGTGTACCCCGACATCCTTGGG

left_200bp	right_200bp
GTTAAATGTGGTACCAGACTCTATCGTTACGATGGTTTAATGCCAGGAGACCCGTCCTATCCAACAATAACACCTAGAGATCCTCGGAATCCACTTATTCGCATACGCGCCAGGGCTGTTGAACCACTTATGCTTCTTATTCCGAGGTAACCATTGCTTAAATTAAAATATTTCAGCTAAACACCTGGTCCGTACCCATA	GATGTCGGGGTACACTTAACTAATACTTATTTACAACAATGAATTAAATATTTTAGAGCACATAAATTTAATTTGTTCATAAAATCCAAAGTGGGGTACGTGTAAGTATATATAGTTTTTTTTATAACTTAAAACTATAATTAATTATTATAAAAATTATATCAACGAGGTCAGAGGTGCTGTTCAAAATGGTTTAGGTC