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CRIMIC CR71343

Gene Pgd

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:2145136..2148566	2D4-2D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATACTCAACATGGACGAGAAGGG	GAACTCCTAACCCAGACTTAGTGG

Images

Construct Design Sequences

sgRNA sequence	CGCTTGCAGCAGGTTTCAATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTGCGCCTACAACCGCACGGTGGCCAAGGTCAAGGAGTTCCTCGCCAATGAGGCTAAGGACACCAAAGTGATTGGAGCCGACTCGCTCGAGGACATGGTCTCCAAGCTGAAGAGCCCCCGGAAGGTCATGCTGCTGGTCAAGGGTGAGTTGCATATCCAAATTCAGCGGCTGGGTAGCGCAGAGCATCGAAAACCCATT	GAAACCTGCTGCAAGCGATCGCTGTGTTGGTGACTCAACTTACATGTGTGCGCGCGTGCTTGTGAATTGGTGAAAAAGTCGAAGCAAAGTAATCATGATGACGATTTTTGCGGCTCATATTCCAATGTGCAAAGGGGAACGAGATAGGATAAGCAGGTGGAAGTAGGAAGAGGAGGGAGCGCGAGAAAACGGTGAAGGGG

 

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