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CRIMIC CR71344

Gene CG1236

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:573648..5738013	83C1-83C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTGTAGTGCTGTAGTCGTATGTC	AGATTGGGAGTATACCCCACG

Images

Construct Design Sequences

sgRNA sequence	ACTTTGTTTACCTACTTGCTTGG
sgRNA2 sequence	CACGGCTTACAGCTCTACTTCGG

 

left_200bp	right_200bp
TGAAAATATAAAAATACGAATAAAAAAATTAAACTATGGCGGTAAAGGCAGTGGTAATAAGTAGCTAAAAATTTAAGCCGATGTACAGGGTAGTCTAAAGTCGAGATCCCCGATGTCTGTGATATTCCTTTTTTAGCGGATTAATGTGTAATGTAAAATTCGGGCACACTTACGGAACCTGTTACTTTGTTTACCTACTT	TAGAGCTGTAAGCCGTGTAATCTCAGCGTACAATGAATTTTTTCCACATCTTTTTAAAAACATCTGTTTGTAATTATGGACAATGTTCTAATTAAATATTTCGTACTTTCAAAGGTATTTTAAAGGTACTGAAATTATTGAAACACTTGTATTACGCCGATGACAGTGCTCGCAATGAACTGATCCGTAAATGGTCGTGG

 

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