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CRIMIC CR71345

Gene Adi1

CG32068

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	III:10662376..10664435	67E6-67E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAAACGAACAGCTGATGCC	TTTCCTCATCCGTGTGCAGGTGTT

Images

Construct Design Sequences

sgRNA sequence	TTAAAAACCTTAAGTGACCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGTTGCTCGTGAAAACCCGTTTAAAACAGTCCAAAATGGTGCAGGTTTGGTATATGGATACGGAGGAAACCGATCAGCGCCTGGAGCACCACCGCAATCCGCCTGCCTACTTGGAACTGGATGATTTGTACCAGAAGACCGGCGTGGAATACTTTAAGGCAAGTGAAAAGCGAAATTGCGACTTTAAAAACCTTAAGTGA	CCTTGGGGGCGAGCTTATCTTGATCATTCACATTTGCAGATCAATGCGGACGAGTACCAGAGCGATAATACCCTCACGGAATTGAGGGCAAAACGTGGCTACACCTACGATGATGAGGCAAGATCGATGTTTTCTTGAAAAACATCTACAGAACTTTAATAAGCTTCGCTCTATTACAGATCACATGCTCGGAAAAGTGC

 

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