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CRIMIC CR71346

Gene Adsl

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:17018780..17021120	89E8-89E8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCTAACAACCTTAAACCTGGGCT	CATTGGAGTCGTGATATGTCAGC

Images

Construct Design Sequences

sgRNA sequence	CGTAATCACAAGGGAAGAGTCGG
sgRNA2 sequence	AGTGGCTGACGCAGTTGATTGGG

 

left_200bp	right_200bp
ATGATATATATAGACCATTGAATAAATTCGAATTAGCTCATTTCCGGTTCCGCTGTTTGAATATAAATATAACGGTCCATTTAGAACTTGGTATATTAGTGTGTGCGTATGGTATTTCTTATTGCCCTATCGCAAGGCGGTCACACTGCCCCGCCACATGTGAGAATATCGCTCAATTAAGCAACAGTTGTTTTCCGACT	CAACTGCGTCAGCCACTGGATGCAGTCGACGTTATCACCTTTTAGACTCATTGGGTCGATGGCAAGCAGACCCCCGGCGTGATTTGTCTTTCTGTTTTTGTATTTTTGCCAGCTTATTTACAATTAAAGGCTAAAATCGAAAAACAAAACCACAAGCCTCGAATCGAACTCGAGTCGACGTTCTTGATCCGAGTAAACAT

 

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