CR71349

CRIMIC CR71349

Gene Gs1

CG2718

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:132077..134472	21B2-21B3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGCATTGCAGGTCTCTCTG	GTGAGATATATGGCAAAGTATTCATGAGG

Images

Construct Design Sequences

sgRNA sequence	TTGGTTAAACAGTCGCTGGTTGG
sgRNA2 sequence	CTCAACGAGTAGGCTGTGAGCGG

left_200bp	right_200bp
CGCAAACGAGATAAAGAAAAACCCGAGGCGCGCGACGAAGCAACGAAAAAAGTGAATGCTGTCGCGCTAACTGTGCTCACAAGACTTCCAAACATCGAATTGGTCGGGTGTCCCCAACAGTCGCTCCGCAGCCCCTGACAGAAACAGAAACGAAAACCGAAAGTACCGCAATCCAAAATTACCAAAAGACCTGTCCAACC	GAGCGGTGCAACAGATGTTTAATTTTTTGTACATTATGTTTTATGGAACTGTGAAGCGTATCCGATCATTTGGTTATATTTACATTGAACTGAAGCAAAATTTAGTTTGCTTGTGCAAAAAAGACAGGAGACCGAGGCACGAAAAGCGTGCGCTTAGAATTTAAGAACGTGGGTGGGTGGCAAGAAGAACTAAGCTAAAG