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CRIMIC CR71350

Gene CG9886

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:2420830..2424647	22E1-22E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAGCAGTGCGTGACATAGCAGC	GGTTTGCCTATTGCCTGCCACATA

Images

Construct Design Sequences

sgRNA sequence	TATGTAAAGGTGCTTCGGAAAGG
sgRNA2 sequence	TATGCAAGTATACAACTAAATGG

 

left_200bp	right_200bp
TAACTTAAATAAATTTGTACATAATATTTGCATAGCTCAATCTACCTCTTAAGTGAAGTTAATTTAATCTGTCCCTAACACTTTGGTGTTTTCGAATCTTCCGCCTGAGGTCGCACTAATAAATATATAATATCTTCAGCTGTTTTGAAAATGATAAGTAAAACTGGAATTTCAGCATCGCTGATTAGGATCGCCCTTTC	AGTTGTATACTTGCATATACATATATGTACATATGTGGGGCAGTTCGTTAATGGAGCTGCTCTTGAACCGCAGACGGGCAATAGTCGTTTAAATTTGTTTTTACGATTTTCCTCTTTAATTTTGCTCTATGAAATTTTTACGACTTTTTTCATTAACATTTTTAATATCCATTCAAATTCAAATAATTTTGTGATCTTTA

 

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