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CRIMIC CR71352

Gene MetRS

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:18846858..18850599	55F7-55F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTTCGGGTCGCTGGTGATGACAT	CGCGATAACCAAGTGTTGGCGAAT

Images

Construct Design Sequences

sgRNA sequence	ACCAAAATAATAGTTTCTCGTGG
sgRNA2 sequence	AAATGCAAACGCAAAACTTATGG

 

left_200bp	right_200bp
AAATCATATATGAATTAGTCTTGTTATTATTAGTCATATTACAATTAATATGTAATAAATATGACCTACTATATTACCTGTTACTTTAATGCCAACTGTTTATTTCAAATTATTTGACATATAAAATAAATAAGTAATCGATAACATCACCGACGGCGTGACAGCTTTTCCATCTGTCCCATCACCAAAATAATAGTTTC	GTTTTGCGTTTGCATTTAACAACCTTTTGTATTTATTCGAAGTATGGCTAAATATTCAGTTAGTAATTTATTGAGTTTGTGAAGAAAAATAAAAAACAATTGACTTTCAATGGAATTGAATTTTAAATTTCGATTTTAGATTACAGTACTAGCTATCGATAAGCATTGTTTACTTATGTGTCACAGTGATTTTTTCAGTA

 

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