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CRIMIC CR71355

Gene Sac1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:1247817..1250451	61F4-61F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGTAGTGGCAAAGAGTTGGC	TCACAACTCCACTTCTTCCCGATC

Images

Construct Design Sequences

sgRNA sequence	TCCCTGCTGTCCATGATTTCCGG
sgRNA2 sequence	GCTAGACGACACTAACGTCATGG

 

left_200bp	right_200bp
AAATCAATAAAGAACGAGTAACCTCCTGATTGTGTGGCTGATTTTGGTGCTGTGCGTGTGTGACAGCGGTGATAGCGACAGTGAATCCCCGATTACAGGAAGTGAGCAGAGAAAGAGGAAGCAGCACTGGCTATAGCTATTAACCCTTATCCCATTTTTAACCCTTAGTCTTCGAAGTGGGCCAGCCACCGCCCCCGGAA	CGTTAGTGTCGTCTAGCAGAGTCACTGGGAACTACCTCTGCAGCTAAACTGTCCCATTGCTCCAAGAAAGAACAATTGTAAACCAATTCTGTAATCGATTCGACTTCCTCTCAATTTCTGTTTTGTTTTATTCTGTTTTTGTTTAGTCTTAAAGCCCCCCCCAGTCCATTCCCTTCTGCAACTTGTTAGTCATTATATAC

 

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