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CRIMIC CR71356

Gene ACC

CG11198

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:7974115..7991862	44A1-44A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAATTCCAAGATCCAACACTAGC	ATAAGCCCAATGCCCCAAGGAC

Images

Construct Design Sequences

sgRNA sequence	ATCTAAGCTCCGTTGGGAGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAATGCGAAAACTAACATATTATTTTCCTACCTGTACATATAGTATATAAAAACCCTGTTGAGTCTAAGTGGCAACAGCTATAAGTAATACAATAGTGTCTGCTTGTTGCAATTTAAGAGTTGTTCGAGTGACAGAAGATTGCCTTCAAAACATTGGATTGCGCACACCTGTAATCTAAAACCCTTCACCATTCCGCCT	CCCAACGGAGCTTAGATTAAACTGCATTCGCAGTACTTTCAAGTGAAAATTCAACCTTAGGTGTTTACTTTCCCTTAGATATAGCAGGGCGCTGGGGGTTAATTAGCAGCCGCCGACGACGACGACTCTTGTTGCACAGTGTGAATTATCTATTTTGTTTTGCTATCCGGGTGTTGCCAATGAACGTCGCAATGACGTCA

 

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