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CRIMIC CR71357

Gene tzn

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:20798645..20800081	77E6-77E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAAGTACAGAATGTTGGCCTC	TTCTCTAGGGTGTTGCTAGACC

Images

Construct Design Sequences

sgRNA sequence	GAACAAAAAGCGTAGATACTGGG
sgRNA2 sequence	AACTTTCGAGGAAATAGATCAGG

 

left_200bp	right_200bp
TTCCGAGGTTCGTCCAGCAATCCGACACTGCTCCTGCGTTCCTGGCGGACGATTCCTCGAGCGGATGCCCCTGGTTTCGGAGCGATTCGGCGGCGTTTTCTGGCTGTGCCCGTCATCTGTCCAAGTCTCGTCAAGCGTAATCTATCCGTACATACGTGTTTAATCTAATCTCTCCCGGTGCTGCAATTTCATTTCCCAGT	CTATTTCCTCGAAAGTTTCGAAACTTGTGATTTAATATTGGATTTTAATGGTTAATAAAATTAACGATGTGTTTGAAGTGCGGACAATGGATTTTTAATTATTCGCAATTTATCGGATTAAAACTTTTTTTGTTTTATTCAAATTATGACATAAATTTATTTCTTCAGCTTTATTTAAAAGAATTTATTTTTGGTATTCT

 

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