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CRIMIC CR71360

Gene Ts

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:2968293..2969638	23C3-23C3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTGTAGAAATTGTACGTCCCT	CGTGTTCTCTCATATGGACGCGAA

Images

Construct Design Sequences

sgRNA sequence	ATCGAGTTCTGCTCGAAAATTGG
sgRNA2 sequence	TGATTTCAAGGTCGCCTACACGG

 

left_200bp	right_200bp
AGATCCACCATCAAAATTCGTATTTTTTTAATAAAGTGAGCTACTGCCCCACGTTCGAACTCTGTGCTCAAATTATTGGCCAAAAACGGTTGGATTTAAATTTTTGAAAATCGCTAGAGGTGATATAACCGGTCGACATCGATAAATGTAATCGCTTGCCTATCGGGTCGAACCGAAGCAATAACTAATTCCCGCCAATT	AGGCGACCTTGAAATCAATAGTTCTGGCTTACTTAAGCAAGCATAATCTTCAACTGAGTAGGTCGCTCACAAATATCTTATTTTCGTTTCAATTTCCAGAAAATTAAACAAAAATTGTTAACCATATATGTATACAATAAATATTATGATAGTCTTTACGGAAACATTTTTTAAACACATCATTTCATAGCTTTATTTGA

 

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