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CRIMIC CR71361

Gene CG18128

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:23718798..23719936	59F6-59F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACAGCTGGATTTCGGAATTCATCAG	GTGTGCACTCTGCACTCTCTACTT

Images

Construct Design Sequences

sgRNA sequence	GGTGAAGTTTCGCACTCATTTGG
sgRNA2 sequence	GGAATCCGAATAGAAGAAGTCGG

 

left_200bp	right_200bp
TTTTGTTTTTAGCGTCATTCGATTCGAGTCACACCTCCTGTTTTCCGCGGACTTAATAAATCGTTGATTTATTTCTGCCGTCATTACATGGTTTAATACTTATTTAAGCCTCTCACACACAAATAGCCCTAAGCTATGCCCTAAAAGTCATCTTTTGAGTATTTTCCAAAAAATTCGAAAAGTGGTGAAGTTTCGCACTC	TCTTCTATTCGGATTCCTATTTTCGGTGCGGCCAGTGGTGCTGCCAACGCGGAAATAGCCACAGGTGCAGCTGTTGGCGCAATCTTCTCGGCGAAGAGTTGTCGCCTAGGGGGGAGGTTTCAAAATGTGTGATCTGAGATGGCCAAGTGGGAAGGGTGAGGTGTGAGGTGTGAGTTGTGAGGTGCGAGTGTGCCCGGTGT

 

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