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CRIMIC CR71365

Gene ATPsynCF6

CG4412

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:23355220..23356064	94E13-94E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCAACTGCCATTGTGAACTGAC	TGCCAACCACAAGATGCAGTTGCT

Images

Construct Design Sequences

sgRNA sequence	GCAGGGATTGCGACAGCATCTGG
sgRNA2 sequence	ACAAAAAAAACCAATAAAATCGG

 

left_200bp	right_200bp
AAGGTGAAAACAAGATTTAAAATACATAAAAATTGATTGAGGGAATACAATTTGAATACAAGATAAAGTTGCTAGCAAAGTCGGCTTGAAAACCGATTGGGAAACGTCGAAACAAACAATTGTATTACATAACCTTTTTAAGGTTACATATGAAGAAGGCCGTTTATTGCTATTCTAACAAGGATCTATCAATTCCAGAT	AATCGGGAAAACAACAAGCTATTCGTATGCGAAAGCCACTAAAAGTGTGTGAATCTCATTGGAGTATAAAGCGCGTCCATTCCTTAACACAGACATTCGTCTTGTGCGACATCATAAACTCAAAACTCATTAAAATCAAATTGAATTCCATTATAAATACAATATATTTAAGCCCTACGATCTAGAAAACAACAACAAAC

 

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