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CRIMIC CR71366

Gene Vha36-3

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:2579091..2580560	3A6-3A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGAATCGAATCCCGAGC	AGTTGGCTGGCGATAGAAACCACA

Images

Construct Design Sequences

sgRNA sequence	TAGTTTAGACTTGGTTGGCGTGG
sgRNA2 sequence	GGTGCAGAATATCCTCGACGAGG

 

left_200bp	right_200bp
CGATCCGATGCGATGCGATGCCTACCCAAGATCGCCGTTCTCTCGCCAGATCCGCTCGATGAGTCTTCATTCGCTGGCCCAGTTTGTCGTCGTGCTGTTCTGTCCGCTGTAGCCCCACTTTGTCGTCTATTTTATTTTCGTTGTGTCGCGTATATTTAGTAAATATTTTGTTGTTGTTATTTATAGTTTAGACTTGGTTG	ACGAGGACGGCGACGAGGATCTGCTCTTCTAGACACACGAACAGCACCCACATGTGTAACCAGCCCCCATCCAGTACTCCACCTGACCTGAGCCCAATTATCACTCAACTTTGCTACCTCACTTCCCACCAAAAGATCACCTCTCGTTATTGTTGTCGTTCGTTGTGATCCCCGGATGTTGTACCACTTGTCGTTTTCTT

 

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