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CRIMIC CR71368

Gene COX8

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:21538859..21539447	78D5-78D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTAATTGTACGCATTGTGACCATAC	CTTCTTCTATCTGTCACTCACGACTC

Images

Construct Design Sequences

sgRNA sequence	GGCGTAAAATCAATGCTGTAAGG
sgRNA2 sequence	TCAATAGTGCCCCTGTTACGGGG

 

left_200bp	right_200bp
ATTGAGTAACTTGATTGACTTAAATTTATTTCTAGGTTGTCAAGAACATTATTTTTAATCAATAAGGTATTTCTAAACAATTATCTGCACCTGAAACACCCTACATTTTCGTTTTGGCTTGCGATAGTTGTGTTTCGCGTAAGACCATCTCTAAAGAAAGGCGAATGACAAATCGTTAAGAAGAAGCATTCTAACCTTAC	ACGGGGAAATAAACTATAGTGCATTGAGCCACAAACTGATTTTATTATTTATCTATGGTCCAGCTGTAACTAAACGTGTGTCTGATATATGGTACTGACATTGTATAAAACGCAGTATGCCAGGTGTAAAGTGGACTCTAAAAGAGGCGGGCGGAATCCTTGATGTTTTAAATTCTTCCGTGTTTAACTTCAAAAACTGG

 

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