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CRIMIC CR71369

Gene ATPsynE

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:14326947..14327735	88B4-88B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAGGCTGCAGTATGTAATACGTC	TGGCAACTACAGTGGGCATCCAAT

Images

Construct Design Sequences

sgRNA sequence	AAGCAAAATTACCAAATTGGTGG
sgRNA2 sequence	AGGAGGAGCATCATCGATAGTGG

 

left_200bp	right_200bp
ATTTTGTTGGGACACTAAACAACAAATAGTCGTCATAAAGGCTTCTTTTAAATTGTTATGATATTTTCCACAATTTCTGCATACTTTTTTAAGCTTGCTGGCAACGCCGCCACCGTACCAATACAACTATCGATAGCCACCTGTCAGTTTTGACAGCTGTTTTAAGGTTATACTGCGCTGCCGTTGCAATCGCTCCACCA	TAGTGGAGCCACCATCTATCGATCACTGACTATCGTTTTTGTGTTTTCCTCTGATGCTTGTGCAAAAAACAGAATGTTCGAAATTAAATGATTGAAACAAAACCATTATGTAAATACTCTCAAGTGTTTCGTGTTTGTGATTAAGTGGTGTTCACTGGAAATATATTTATATTAGCATAGTGTTTGGATAGGAGAGGATT

 

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