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CRIMIC CR71370

Gene NP15.6

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:19027368..19028015	91E4-91E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTCCGAACCAATTGCTCTGGAA	CAGTTCTGGAGGCTCTGCATC

Images

Construct Design Sequences

sgRNA sequence	AACAGCGCAGACATCGCGTTGGG
sgRNA2 sequence	TTACATAACAGACTAAGTTTAGG

 

left_200bp	right_200bp
TTATTTCGAGCAAAGTTTCTAGGAATTCAAATTAAAACGGTTCTAGGTAAAATACCGAAAAAATACCGCATCGAGTGGCTACCCTCTCGGCCAGCTGACAAATTGCAGCAAACGTTCACACTGCGCAGCTGTCAGAAGTCCAGCGATCAAGGGAAAACTCGATTATTTACGAAATTTGCGACTGAAATTTCTTCCCCAAC	CTTAGTCTGTTATGTAATGCGAATGCGAAGTGCGGTCAGTGCGAGTCCGCTCATAATCCGTTAATAAATCTGCAAGTCTCGCCGTGGACACAGCTGCTTTTTAGCCCTCGGATGATTAATTTTGATCAAATATGTTACATTTTCAACCATCGCGTTTGAGGTTAGGTGTTCAAAACATAAACAAGCTAGTACGTACGCAT

 

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