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CRIMIC CR71371

Gene ND-19

CG3683

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:24664398..24665439	60D13-60D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGATCTAAATCAGCTGTTGTGTTG	TCTTCACCTTGCGGAAGAAGTC

Images

Construct Design Sequences

sgRNA sequence	TCCTCCTGATAGTAATGCTTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTGATCGCACAGGACTGCAACGCTATTATGGTCATCACCAACAACACAACACTGCCCGAGGAGTCGGAGCTGAACGTCCAGGAGCTAAATCTATCGTCGGCGGCCTTACGGGCAGGCGCCTTCCATTTGGGCAAGCAATGCGAGCAGGCCAATAATGTTAGTACATCCAGATTGCAGGGGAATCCTCCTGATAGTAATG	CTTAGGTGAAAAGCGCCTTAAAGTGAAGCTGGGAGCCGCTGCCTCTTTAAAATCCGAGCGGCTTAGTTATATAACCTAACCTCAAAAAGCACCTTCCCCGTTTGTTTACCTTTTCCTTCGTGTAATTTCAGGAGTTTATGCTGTGCCGGCAGGAGCTGGATGACCCGCGAGCCTGTTTGGCGGAGGGCAAAGCCGTGACC

 

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