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CRIMIC CR71372

Gene Dad1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8383612..8384137	29B4-29B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCGGTACAGTGAAAATCGGATAG	CGGATGCCTTAAAGCACACATCGA

Images

Construct Design Sequences

sgRNA sequence	GATATTTGTCCGGATATTTAAGG
sgRNA2 sequence	AAGTCGGCGAAACCGCGTTCCGG

 

left_200bp	right_200bp
AACTAAAACACAAAAATTTTTTAATATATAAACCTATTCTTTATTAAACTTCAAGGTATTGAGTGCCCACTGTACTTGCGCCTTCATCGATAAAGCTATCAGTATTATTTTCAAACTAGACGAACGGTCCTACTTTTGAGTTCTCTCCCAATTTGTGACGTGTCTGCGATTAATTTGTTAAAAAGCGCCTTTATCCTTAA	CGCGGTTTCGCCGACTTCATCTTCGCTCATGTGATCCTTCATCTGGTGGTCATGAACTTCATCGGTTAACAAAGCTACAAACATTCTGATATTCTCGGATATATTTATAAATAAAATGTAACTCTAATTGTATGTTCTCCTATATGCGCCGAAAAAATCGAAGGGTCCTGGTTCCCAATCCAAGTGGAGTACTGGTTCCT

 

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