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CRIMIC CR71373

Gene CG33303

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10373369..10375757	31D11-31D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTAGGTCAGTACTTGATAAGTGCTTCG	CCGAATTTGGGCATATGTGCAC

Images

Construct Design Sequences

sgRNA sequence	TGGAAAATCTCGAAGCGTTAGGG
sgRNA2 sequence	TACTTATTCCTTATGCGCATGGG

 

left_200bp	right_200bp
TTGATGCAAAATAAGCTTTATTTAGAAGTGCTATTATTTTGGTTACTGCCCTTTATATATTTTTTCTGTTATAAGAATAAAAATTAAAATATTTATGGAATACCCCTGATAATTCTGCGCAGTGGCTGGTAAAAGTACGAATGCGTGACACGGTCACGCTGAGCCGCTTTGCCGGCTGAATGGTCTTAAATATGCCCTAA	CGCATAAGGAATAAGTAAATCTTTAGATTTCCACTAAATTTAGTCACTCATCAGATTGTAAAAGATCAAAATGAAACAAAACTACTATTTTGCAAATAACAAACGAAAATAAATAAAAGCTTGCACTTGATGGCGTGAAATTTAATCGTAAAAAGGTAATGTTTCTTCTAGCTAAAAATGAAATAAATTCCAAAACTAGA

 

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