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CRIMIC CR71374

Gene Alg13

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:29137970..29138573	98F12-98F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAGCACAATGAGCGAGATG	CAACCAAAGCACGTTGAGAGTCTC

Images

Construct Design Sequences

sgRNA sequence	GAACGTCGTATGAGTAACGCCGG
sgRNA2 sequence	CACCAAAAGAAGCTTGCATTTGG

 

left_200bp	right_200bp
GAGCAAAATAAACTGAGCGCTGTGAAAGCGGAATACTTACGTTTAAAATTGAATTTAAGCATTTTGCTTAAACAAGTATTGCTTAAAACTATTTCACTACTCTTTTCACTTATTTACTGCACTCCTAGTAGATAAAGTAACGGTTATGGCCGGTGTTTCGAGCTTCCAGGCTGCCATTTATTTGAACGTCGTATGAGTAA	GCAAGCTTCTTTTGGTGCCAACCCCAAGGGGTTAGCCTTTTCTATTTATTTGAGTTTACAAGTATAGTTAATAAACACGAGAACGTCTACGAAGAACATTAAATATTGTTAGCAATCTAACGTTTGCTGGGGCTATACGATATACATACATAGATAAATTCACGACAATTAAACATTAAAACGTACATATATGTTTGTTA

 

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