CR71376

CRIMIC CR71376

Gene GluRS-m

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:16415292..16417177	72F1-72F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACCTACCTGAGCCGTGTAATAC	ACCAATCGCGACTTTAGCTAAGCG

Images

Construct Design Sequences

sgRNA sequence	GGTAAACAAAAAACCGGCATAGG
sgRNA2 sequence	TTTGAGTCGTTCGAGAGTGACGG

left_200bp	right_200bp
TTACGATTTACTGCCTTTTCTGCATTCATAATATTATGAAATTCAAAGAAAACAATTAAGAAAACAATGAAAAATATCCAATTTAAAATTTTCAATCAGAAAAATTCAAATAAGACACCTTGCTGTTATGCCACTGTTAAGCCATATGCGAGTTAACAGTTCTAGTTTCGATCAGCTGTTTGGGGTAAACAAAAAACCGG	CTCTCGAACGACTCAAAGAAGCTCTGCCCAAGGAAAGTCTGCATTTAGAAGAACATCAGTGATTTGGTTAAGTTCTGTTGTATTTGTATGTATATATAATAGATTAATTTATATATTGATAACTTTTGCAATTTGAGAATAAGATCAATGAGTAGACACCACATGTAGAGTCAAAATTGTGTATGGTTAATCTGCATCAG