CR71377

CRIMIC CR71377

Gene Nfs1

CG12264

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:11999654..12001244	33B5-33B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGAATTCGTAACTTAAGTTCGCTGAATG	GCAGTTGTACTTGCACATGTTCGC

Images

Construct Design Sequences

sgRNA sequence	TGATGGAATCCTAGAAATTCTGG
sgRNA2 sequence	AAAGTGTGGTGTAACAGTAACGG

left_200bp	right_200bp
GCGAATATATCTGAAAAATATTGGTTTCTTGCACAGATGCTATTCATTTTTCATATGTTCAAAGAAATTCAGAAACTTGCTTCTGCATAATCGAATCAGATAATTTTAAAGTGAATTCCGGAGTCCTTTGGTATATCTCGTGCCGCCGAATACGGTCCCACTGATTGTGAAATCCGTGATAACGAATTTTAACACCAGAA	CTGTTACACCACACTTTGAAAGCGAAGGGAAAATAAATTTGTTGGAAGAGTATCTAGGTTTATTTGTACACAAAACCACTTATAACTAAGCTTAGTCCGAACGAATGTGCTTGTGTAAGAGGTGAGGCAAGAGAGACCCCAAAAAGCGAAAACGGCTCAAAGTGCGACGGCCAATGATATGCAAATGATGCAATAATTTG