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CRIMIC CR71378

Gene Coq3

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:21157433..21158611

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACTGAAAAGAGTGCTCCATTCC	AAGGAGGTATCCGACCAAGACATG

Images

Construct Design Sequences

sgRNA sequence	GGGCGTAGCCAGGTGCATTAAGG
sgRNA2 sequence	CCAGATGTGCTACGCCTTGCAGG

 

left_200bp	right_200bp
TTACATTTCACAACTGGGAACTGACGTGTGTGGAGAACCCCATCTCCTTTATTTTCTTTATATTCATTTTCTTTATTAATATTTAAATTTTTACCTGAACAAAAGTACCTGAAAGTACTATAATGCCTTACAATATGGGTGTTTAAAAATGACAAATAATAGTTATTGTTTAATATTCAAGAATTGTCAATCCCGCTTAATGCACCTGGCTACGCCCATGCTTTCGCCGGGCAGCAAAACAAAGTCAGCTGTTTTTGTTTATCAACGTTTGTTTTTCTCGCAAAA	TGCAGGCGGTGAAACAAGCACAGCGCGAGTACAATTGAATTCGTATATTTATTTAACTAAAAGCTAAGTCACGAGTGTAACAACTAAGTAGTAAACTATTCGTCACCCGAGCGTGGTTTCTTAAAGTACTGCTTCTTCATGGCATGCGGACTATCACTATCTCCGCCGATTTGCTTCCTTTTAAAGTCCTTTTTGGTGTT

 

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