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CRIMIC CR71379

Gene Cox10

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10331471..10333129	31D9-31D9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGAAACTGAAAATGTCTCTGAGTTTTACC	CTGGAATCTGAAGAAGAGATGCC

Images

Construct Design Sequences

sgRNA sequence	TCACTTATTAGTTAGGTCTACGG
sgRNA2 sequence	AGGAAACACGTACCCCTGTGCGG

 

left_200bp	right_200bp
TTGCTATGTTTTTTTCAAAGTTCTTAAAAAATCCCAATCCCAACAATTTATGAATACCTCTGATTAAATTTTTTGCACTCAGTATTTTTCTCATTTTGAGATATGGTAACACTTAAGCACAATGTGATTTAAAAAACCATAACATTGTATATAAAAGTAATAGAAATCCAAACACAACTAGAAAAATGAAATAACCGTAG	GTGCGGCAAATATATGTTGCGCCTAATAAATGACACGACATCCTTGAAAAATATGCGGTGTAACCAATCCGGCGCTGAATACAAATAAATTTAAAATATACAATTTGGCATGGAATCTTAAGCCGTACAGTAATTTAAATTGCAAGATTCTAAGTATGACCTTATTATATTGTTCTTTGAGCACTTGTTTCAAAAAAAGC

 

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