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CRIMIC CR71381

Gene Mad

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:3146056..3159643	23D3-23D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTCGATAGCCTACTACGAGCTAA	ACGGTGCTCGGATGGAATC

Images

Construct Design Sequences

sgRNA sequence	TAATAACTAAGATTACACATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGTGATCGTTGACGGTTTCACGAATCCTTCCAACAACTCGGACCGCTGCTGTCTCGGCCAGCTGAGCAACGTGAACAGGAATAGCACCATCGAGAACACACGCCGTCATATAGGTGAGTCGATTTTAGCACTTAAATTAACTTACTCACAGAGATTTCCCATCAGCAACTATCTTAAAGTGGTTAATAACTAAGATTACA	CATTGGTAATCTTAAATTAGAAAGCGACTTGTGTTTATCAGTTATAAAAATAGGCATTGAGTAGAGTAGAAAGCTATGGACTTCAAGAATCCAACTACCTGTCTTTTTAACTCAAAATGTTTCTTCTTCCCCAGGCAAGGGTGTTCATTTATACTATGTGACCGGCGAGGTCTACGCCGAATGCCTATCCGACTCCGCCA

 

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