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CRIMIC CR71382

Gene Pur-alpha

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	4:558016..567257	102C5-102C		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AACTATATGCTGTCGTGTTGAGG
sgRNA2 sequence	TTATATAACTTAGTAAATCACGG

 

left_200bp	right_200bp
TCCTACGAAATTTACTCATATGATTTAGGTATTAAAGGAAGTTAGTAAACAAAAACGAATTTACAGAAAAAAAAACAAACACAGCTGCTGTGCTGCTACTCTACGATATTAATCATAATTATCTATATTATATTTAAAGCAGGGGAATGTTGTACACCGGACAAAAAGAATGGTTATACTATTAACTATATGCTGTCGTG	TTTACTAAGTTATATAAGAAGGCGCGAAAATAAAATTTTTAATGGCGATTGACCAACCAATATATGTAAAGCTTGTAATGTCTGACATGGCTAAATTCTAAATCATGCGACTAATTAAGCTTAATGACAAACAAATATATTTATTTTGTAGCATTTAGCCCAATATGTTTTAGTATTCTATAAATTCTATATATAAATTA

 

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